Extended Biocatalytic Halogenation Cascades Involving a Single‐Polypeptide Regeneration System for Diffusible FADH₂

We constructed a bifunctional fusion protein for FADH2 regeneration and successfully coexpressed it with different flavin-dependent halogenases, as well dioxygenase that converts 6-chlorotryptophan to 4-Cl-Kynurenine. The figure is still life of laboratory bench, single oversized E. coli bacterium hovering over an Erlenmeyer flask. Cell disruption depicted in visual analogy cracking egg the lysate appearing like yolk. flask contains all necessary enzymes biocatalytic cascade described paper rendered ribbon structures coloring consistent paper. Shown permanent marker notes on bench surface are key reaction scheme “ToDo-list” checks off some important goals research work. More information can be found Research article by N. Montua, Sewald.